Alterations between Autophagy and Apoptosis in Alveolar Bone Mesenchymal Stem Cells under Orthodontic Force and Their Effects on Osteogenesis

The aim was to investigate changes between autophagy and apoptosis in alveolar bone mesenchymal stem cells (BMSCs) under orthodontic pressure the effect on osteogenesis BMSCs. Twenty-four beagle canine teeth were used as experimental models. control group received no treatment, while groups, canines moved distally with 150 g of force for 7 days, 14 days 28 days. Bone remodeling detected by H&E staining, analyzed immunohistochemistry. 3-MA Z-VAD-FMK modulate apoptosis. Transmission electron microscopy western blotting analyze BMSCs, their ALP alizarin red osteogenic gene analysis. Compared group, we observed significant distal movement each distance loading time extended. In side clearly absorbed. On side, positive LC3 after whereas caspase-3 number autophagosomes conversion LC3-II/LC3-I gradually decreased, nevertheless, apoptotic bodies expression increased gradually. activity, mineralized nodule formation, BMSCs decreased. Finally, down-regulation inhibited restriction led adverse consequences. Collectively, these data indicate that decreased extended, contrast, extent increased; differentiation positively correlated but negatively